CLICK VLP

SPLITTERA for VLPs functioning

Why SPLITTERA? CLICK VLP

SPLITTERA is a simple, fast, efficient and cost effective universal tool that allows conjugation of VLPs with antigens of any size. It creates a new covalent bond between the structural proteins of the VLP and the antigens of interest, leading to the functionalization of VLPs by antigen display. SPLITTERA improves the possibilities of classical VLPs overcoming the current limits in antigen size. Moreover, it is compatible with antigens produced in any host.

Key Advantages

  • UNIVERSAL:
    • Conjugation with antigens of any size that can be produced in any host.
    • Standard conjugation reaction, common for all antigens: suitable as vaccine platform.
    • Multiantigenic VLPs: up to 4 different antigens.

 

  • HOMOGENEITY:
    • Site-specific conjugation for optimal orientation of antigens.
    • Covalent conjugation.
    • Antigens maintain native conformation.

 

  • FAST AND SIMPLE:
    • Standard manufacturing, no need for complex downstream setup for each VLP-Ag.
    • Extremely fast conjugation, high reaction yield, 100% specificity.

 

  • ADDITIONAL FEATURES:
    • Allows affinity purification of tag-less VLPs.
    • Mild conjugation reaction, active in broad range of pH, temperature, concentration of detergents and urea.

VLPs functioning

SPLITTERA split inteins are not present in the final product, leaving a scar of maximum 6 aminoacids. Consequently, VLPs display antigens maintaining their properties, making it possible to use SPLITTERA to conjugate antigens of any size.

  • Antigen (Ag) in fusion with one intein moiety is produced recombinantly in the host of choice.
  • VLP in fusion with the other intein moiety is produced recombinantly in the appropriate host
  • Protein trans-splicing (PTS) occurs when inteins interact.
  • VLP surface i s covalently decorated with Ag